“Genetic Diversity analysis among various Biotic and Abiotic Stress Resistant Rice Genotypes using Hyper-variable SSR Markers”

Abstract

The objective of present study was to evaluate the genetic polymorphism and identification of diverse parents among the 11 rice genotypes using Hyper-variable Simple Sequence Repeat marker (HvSSR). These microsatellite markers could serve as a powerful tool in selecting genetically diverse germplasms, to execute efficient selection in highly segregating generations. Out of 26 HvSSR markers, 22 markers were amplified in all the eleven rice genotypes. A total of 54 alleles were detected with 22 markers with an average of 2 alleles per marker pair. Polymorphic information content (PIC) value of SSR markers ranged from 0.89 (HvSSR2-50) to 0.583 (HvSSR11-21) with an average of 0.73. The UPGMA clustering placed biotic resistant  rice genotype namely BM-71 (BPH) and MIL-12 (Blast)  in cluster I, while other biotic resistant rice genotypes namely, NLR-145 (Blast) and one abiotic resistant (P use efficiency) rice variety namely Swarna  in cluster II. Cluster II also consists of susceptible genotypes namely WGL-44, WGL-14, WGL-962, KNM-118, WGL-32100, Bhadrakali and MTU-1121 with varied level of % of similarity between the genotypes. Hybridization among these diverse parents may helpful to obtain better genotypes with biotic and abiotic stress resistance. Results obtained from the present investigation would be highly useful in rice breeding programs and may be used for further crop improvement using advance marker systems.